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07-1586

Sigma-Aldrich

Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564)

from rabbit, purified by affinity chromatography

Synonym(s):

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein, MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

Specificity

This antibody recognizes the C-terminal VHL recognition site of HIF-1-alpha hydroxylated at Pro564.

Immunogen

Epitope: C-terminal VHL recognition site
KLH-conjugated linear peptide corresponding to the C-terminal VHL recognition site of human HIF-1-alpha, hydroxyproline (Pro564).

Application

Anti-HIF-1-alpha Antibody, hydroxyproline (Pro564) is a rabbit polyclonal antibody for detection of HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha & has been validated in WB, PIA.
Peptide Inhibition Analysis: A representative lot of this antibody was used for peptide blocking using an in vitro transcribed and translated HIF-1-alpha protein.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blot using in vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected in vitro transcribed and translated HIF-1-alpha protein treated with FeCl2.

Target description

~92 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
In vitro transcribed and translated HIF-1-alpha protein untreated and treated with FeCl2

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ying Liu et al.
Autophagy, 19(9), 2443-2463 (2023-02-22)
Mitophagy, which selectively eliminates the dysfunctional and excess mitochondria by autophagy, is crucial for cellular homeostasis under stresses such as hypoxia. Dysregulation of mitophagy has been increasingly linked to many disorders including neurodegenerative disease and cancer. Triple-negative breast cancer (TNBC)

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