SRP4702

CTGF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(e): connective tissue growth factor
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD07370810

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC), ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 501.0-2.0 μg/mL ED₅₀
• mol wt: ~11.2 kDa
• packaging: pkg of 20 μg
• impurities: endotoxin, tested
• NCBI accession no.: NM_001901
• UniProt accession no.: P29279
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CTGF(1490)

Beschreibung

General description

The gene CTGF (connective tissue growth factor) is mapped to human chromosome 6q23.2. CTGF is a member of the CCN (Cyr61, CTGF , Nov) family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. The protein has an N-terminal secretory signal peptide and four domains (an insulin-like growth factor-binding protein domain, a Von Willebrand factor domain, a thrombospondin type-1 repeat module, and a CT). It is expressed in various tissues, such as connective tissue, heart, brain, kidney, lung, liver, muscle, pancreas gland and placenta. Recombinant human CTGF is a 11.2Da protein of 98 amino acid residues.

Biochem/physiol Actions

CTGF (connective tissue growth factor) stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells. It binds to IGF (insulin growth factor), TGF (transforming growth factor) β1 and BMP (bone morphogenetic protein)-4. CTGF plays a crucial role in the progression of diabetic nephropathy. It can work as an oncoprotein in glioma and melanoma. However, it works as a tumor suppressor in lung cancer and colon cancer.

Physical form

Sterile filtered and Lyophilized without additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable