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S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Synonym(e):

Saline-sodium citrate buffer

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About This Item

UNSPSC-Code:
41105319
NACRES:
NA.25

Form

powder blend

pH-Wert

6.8-7.2 (20-25 °C, 263 mg/L in water)

Löslichkeit

water: 263 mg/mL, clear, colorless

Fremdaktivität

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

Lagertemp.

room temp

Allgemeine Beschreibung

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Anwendung

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Leistungsmerkmale und Vorteile

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Komponenten

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
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Aline V Probst et al.
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