P9474

Pyruvate Decarboxylase from baker′s yeast (S. cerevisiae)

ammonium sulfate suspension, 5.0-20.0 units/mg protein (biuret)

• Synonym(e): 2-Oxo-acid carboxy-lyase
• CAS-Nummer: 9001-04-1
• EG-Nummer: 4.1.1.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00132144
• Specific activity: 5.0-20.0 units/mg protein (biuret)

Eigenschaften

• form: ammonium sulfate suspension
• specific activity: 5.0-20.0 units/mg protein (biuret)
• storage temp.: 2-8°C

Beschreibung

General description

Pyruvate decarboxylase (PDC) usually appear in plant seeds at the time of germination, especially when the plant embryo is totally covered by an oxygen-impermeable testa.

Application

Pyruvate Decarboxylase from baker′s yeast (S. cerevisiae) has been used to evaluate the power of systematic identification of meaningful metabolic enzyme regulation (SIMMER) for finding unknown yeast regulatory interactions.

Pyruvate decarboxylase (PDC) is used to study residues involved in thiamine pyrophosphate (TPP) binding[1]. It is used to study the regulation of fermentation pathways in plant species[2].

Biochem/physiol Actions

Pyruvate decarboxylase (PDC) actively participates in the anaerobic metabolism of several bacteria, yeast and plant seeds.

Pyruvate decarboxylase (PDC) is a homotetrameric enzyme that catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide in the cytoplasm. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. PDC contains a β-α-β structure, yielding parallel β-sheets[3].

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ pH 6.5, stabilized with 5% glycerol, 5 mM potassium phosphate, 1 mM magnesium acetate, 0.5 mM EDTA, and 25 μM cocarboxylase.

Preparation Note

Isolated without the use of heavy metals.

Other Notes

One unit will convert 1.0 μmole of pyruvate to acetaldehyde per min at pH 6.0 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable