P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

• Synonym(e): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS-Nummer: 37288-19-0
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-638-2
• MDL number: MFCD00131896
• EG-Nummer: 4.6.1.13 (BRENDA, IUBMB)
• Specific activity: ≥1,000 units/mg protein (Lowry)
• Biological source: Bacillus sp. (Bacillus cereus)

Eigenschaften

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: ≥1,000 units/mg protein (Lowry)
• mol wt: 28 kDa
• foreign activity: Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein
• storage temp.: 2-8°C
• Gene Information: Bacillus cereus E33L ... BCZK3513(3026815)

Beschreibung

General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

• in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
• to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
• to cleave immunolabeled HeLa cells

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)