M7933
Anti-phospho-MAP Kinase Kinase 3/6 (pSer189/207) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
unconjugated
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
buffered aqueous glycerol solution
Speziesreaktivität
human, rat, mouse
Methode(n)
immunoprecipitation (IP): 1:250
western blot (chemiluminescent): 1:1,000 using anisomycin-treated NIH-3T3 cell extract
UniProt-Hinterlegungsnummer
Versandbedingung
wet ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
phosphorylation (pSer189/pSer207)
Angaben zum Gen
human ... MAP2K3(5606)
mouse ... Map2k3(26397)
rat ... Map2k3(303200)
Allgemeine Beschreibung
Activation of MAPK signalling requires the phosphorylation of upstream MAPK kinases or MKKs. MKK3, MKK6 are activate the p38 MAPK isoforms, p38α, p38δ, with the probable involvement of MKK4. MEKKs do not activate the ERK or SAPK/JNK MAPK signalling. MEKK3/6 also mediate the stimulation of pro-α1(l) collagen by TGF-β1 signalling. TGF-β1 signaling stimulates VEGF via activation of p38α and p38β by MEKK3, in murine mesangial cells. Both MEKK3 and MEKK6 are activated in response to cellular stress and inflammatory cytokines. The phosphorylation sites are Ser189 and Thr193 for MKK3 and Ser207 and Thr211 for MKK6.
Anti-phospho-MAP Kinase Kinase 3/6 (MKK3/6) (phosphoserine 189/207) detects MKK3/MKK6 only when phosphorylated at Ser189/207. It does not cross-react with the corresponding phosphorylated MEK, MEK2, and MKK4/Sek1.
Anti-phospho-MAP Kinase Kinase 3/6 (MKK3/6) (phosphoserine 189/207) detects MKK3/MKK6 only when phosphorylated at Ser189/207. It does not cross-react with the corresponding phosphorylated MEK, MEK2, and MKK4/Sek1.
Spezifität
The antibody detects MKK3/6 only when phosphorylated at Ser189/207. It does not react with non-phosphorylated MKK3 or MKK6 or phosphorylated MKK4/SEK1, MEK or MEK-2.
Immunogen
synthetic phospho-Ser189 peptide corresponding to residues around Ser189/207 of human MKK3.
Anwendung
Anti-phospho-MAP Kinase Kinase 3/6 (MKK3/6) (phosphoserine 189/207) may be used at a working dilution of 1:1000 for detection by immunoblotting in NIH-3T3 cells treated with anisomycin. The antibody may be used in immunoblotting application in rat hepatoma Fao and human hepatoma HepG2 cells. The antibody is also suitable for immunoprecipitation at a working dilution of 1:250.
Physikalische Form
Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Bozena Kaminska et al.
Acta biochimica Polonica, 52(2), 329-337 (2005-07-02)
Transforming growth factor beta (TGF-beta) is a multifunctional cytokine involved in the regulation of cell proliferation, differentiation and survival/or apoptosis of many cells. Knock-out experiments in mice for the three isoforms of TGF-beta have demonstrated their importance in regulating inflammation
J Raingeaud et al.
Molecular and cellular biology, 16(3), 1247-1255 (1996-03-01)
The p38 mitogen-activated protein (MAP) kinase signal transduction pathway is activated by proinflammatory cytokines and environmental stress. The detection of p38 MAP kinase in the nucleus of activated cells suggests that p38 MAP kinase can mediate signaling to the nucleus.
Lin Wang et al.
The Journal of biological chemistry, 279(32), 33213-33219 (2004-05-15)
Transforming growth factor-beta1 (TGF-beta1) is a potent inducer of extracellular matrix synthesis leading to progressive glomerular fibrosis. The intracellular signaling mechanisms involved in this process remain incompletely understood. The p38 mitogen-activated protein kinase (MAPK) is a major stress signal transducing
J Han et al.
The Journal of biological chemistry, 271(6), 2886-2891 (1996-02-09)
Mitogen-activated protein (MAP) kinases require dual phosphorylation on threonine and tyrosine residues in order to gain enzymatic activity. This activation is carried out by a family of enzymes known as MAP kinase kinases (MKKs or MEKs). It appears that there
Rina Hemi et al.
Diabetes, 60(4), 1134-1145 (2011-03-10)
Stress stimuli such as tumor necrosis factor (TNF) have been shown to induce insulin receptor substrate (IRS)-1 serine phosphorylation and insulin resistance by transactivation of ErbB receptors. We aimed at elucidating the potential role of p38 mitogen-activated protein kinase (p38MAPK)
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