J2128

Anti-phospho-c-Jun (pSer⁶³) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD01092289
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, FACS, WB CL
• Citations: 7

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen 48 kDa
• species reactivity: rat, mouse, human
• technique(s): flow cytometry: 1:200, microarray: suitable, western blot (chemiluminescent): 1:1,000 using extract of NIH3T3 anisomycin or UV-treated cells
• UniProt accession no.: P05412
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer63)
• Gene Information: human ... JUN(3725) ; mouse ... Jun(16476)

Beschreibung

Immunogen

synthetic phosphoserine⁶³ peptide corresponding to residues around Ser⁶³ of human c-Jun, conjugated to KLH.

Application

Anti-phospho-c-Jun (pSer63) antibody produced in rabbit has been used in microarray analysis to identify the changes in the level of cellular proteins in cells inoculated with Equine influenza virus.

Biochem/physiol Actions

The antibody does not react with the corresponding phosphorylated form of JunD or JunB.

The protooncogene c-Jun encodes a member of the jun family that forms a component of the transcription factor AP-1 (Activator Protein-1). It dimerizes with a member of the Fos family via the leucine-zipper motif to form AP-1, a factor involved in binding to TPA (12-O-tetradecanoylphorbol 13-acetate) response element (TRE) of various genes that include human collagenase, metallothionein IIa, stromelysin, interleukin 2, SV40 and polyoma. The dimers formed are involved in the activation of AP-1 dependent genes. AP-1 regulates the transcription of mammalian DDI (DNA-damage-inducible) genes.

Physical form

Solution in 10 mM HEPES sodium, pH 7.5, containing 150 mM sodium chloride, 100 μg/mL bovine serum albumin, and 50% glycerol.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids