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F4137

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL-Nummer:
MFCD00162224
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

FITC conjugate

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Lagerbedingungen

protect from light

Methode(n)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
indirect immunofluorescence: 1:200

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .

Spezifität

Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.

Immunogen

Chicken IgY (IgG)
Purified chicken IgY (IgG)

Anwendung

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used:
  • in indirect immunofluorescence
  • in western blotting
  • in immunocytochemistry

Biochem./physiol. Wirkung

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing IBV-S1 and chicken IFN gamma
Wang Y F, et al.
Vaccine, 27(50), 7046-7052 (2009)
Aymn T Abbas et al.
Vaccines, 8(4) (2020-11-04)
The Middle East respiratory syndrome coronavirus (MERS-CoV) was identified in 2012 and causes severe and often fatal acute respiratory illness in humans. No approved prophylactic and therapeutic interventions are currently available. In this study, we have developed egg yolk antibodies
Ori Bar-Nur et al.
Nature biotechnology, 33(7), 761-768 (2015-06-23)
Brief expression of pluripotency-associated factors such as Oct4, Klf4, Sox2 and c-Myc (OKSM), in combination with differentiation-inducing signals, has been reported to trigger transdifferentiation of fibroblasts into other cell types. Here we show that OKSM expression in mouse fibroblasts gives
Beatriz Maria de Almeida Braz et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 29(4), e014120-e014120 (2020-10-22)
Toxoplasmosis is a parasitic disease, which is prevalent across the world and has a strong environmental component in its transmission chain. The present study determined the seroprevalence and factors related to Toxoplasma gondii infection among free-range chickens from an Atlantic
Lineage conversion induced by pluripotency factors involves transient passage through an iPSC stage
Bar-Nur O, et al.
Nature Biotechnology, 33(7), 761-761 (2015)
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