Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

F4042

Sigma-Aldrich

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-ddddk, Anti-dykddddk

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

MDL-Nummer:
MFCD00282246
UNSPSC-Code:
12352203
NACRES:
NA.32

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

M5, monoclonal

Form

buffered aqueous solution

Aufgereinigt durch

using Protein A

Speziesreaktivität

all

Konzentration

2-5 mg/mL

Methode(n)

western blot (chemiluminescent): 10 μg/mL

Isotyp

IgG1

Immunogene Sequenz

DYKDDDDK

Versandbedingung

dry ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Anwendung

Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Physikalische Form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Angaben zur Herstellung

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Sonstige Hinweise

Antibody is not calcium dependent.

Rechtliche Hinweise

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Produkt-Auswahlhilfe.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Chih-Chao Liang et al.
Nature communications, 7, 12124-12124 (2016-07-14)
The Fanconi anaemia (FA) pathway is important for the repair of DNA interstrand crosslinks (ICL). The FANCD2-FANCI complex is central to the pathway, and localizes to ICLs dependent on its monoubiquitination. It has remained elusive whether the complex is recruited
Franz Oswald et al.
Nucleic acids research, 44(10), 4703-4720 (2016-02-26)
The transcriptional shift from repression to activation of target genes is crucial for the fidelity of Notch responses through incompletely understood mechanisms that likely involve chromatin-based control. To activate silenced genes, repressive chromatin marks are removed and active marks must
CCAAT/enhancer-binding protein beta regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes
Ushijima T, et al.
The Journal of Biological Chemistry, 289(5), 2852-2863 (2014)
Bader Almuzzaini et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(8), 2860-2873 (2016-04-30)
Actin and nuclear myosin 1 (NM1) are regulators of transcription and chromatin organization. Using a genome-wide approach, we report here that β-actin binds intergenic and genic regions across the mammalian genome, associated with both protein-coding and rRNA genes. Within the
DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism
Furman C, et al.
The Journal of Biological Chemistry, 277(10), 7962-7969 (2002)
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.