F0628

Ferredoxin-NADP⁺ Reductase from Spinacia oleracea (spinach)

lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

• Synonym(e): Ferridoxin-NADP⁺ oxidoreductase
• CAS-Nummer: 9029-33-8
• EG-Nummer: 1.18.1.2 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00131050
• Specific activity: ≥15 units/mg solid

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥15 units/mg solid
• foreign activity: NADH diaphorase activity ≤10 units/mg solid
• storage temp.: −20°C

Beschreibung

General description

Ferredoxin-NADP⁺ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.

Application

Ferredoxin-NADP⁺ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.

Ferredoxin-NADP⁺ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.

Biochem/physiol Actions

Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

Other Notes

One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)