E2156

Anti-EGF Receptor antibody, Mouse monoclonal

clone 225, purified from hybridoma cell culture

• Synonym(e): Anti-EGFR, Anti-Epidermal Growth Factor Receptor
• MDL-Nummer: MFCD00162282
• UNSPSC-Code: 51111800
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 225, monoclonal
• Form: buffered aqueous solution
• Speziesreaktivität: human
• Konzentration: ~1.5 mg/mL
• Methode(n): immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P00533
• Anwendung(en): research pathology
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... EGFR(1956)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.

Immunogen

partially purified EGF receptors from human A-431 cells.

Anwendung

Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.

Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).

Biochem./physiol. Wirkung

EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.

EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .

Physikalische Form

0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

Rechtliche Hinweise

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable