I am extremely dissatisfied with the Proline Specific Endopeptidase from Flavobacterium sp. Despite strictly following the enzyme activity assay instructions to cleave Cytochrome C at a concentration of 1 mg/ml in PBS, with pH adjustments tried at both 6.5 and 7.0, the results were utterly disappointing. I experimented with enzyme to substrate ratios of 1:20, 1:50, and 1:100, incubating each mixture at 37°C for over 20 hours. Despite these efforts, mass spectrometry analysis revealed absolutely no cleavage, indicating that the enzyme activity was non-existent. This product is completely ineffective, and I strongly advise against wasting time and resources on it.
Fortfahren mit
E1411
Proline Specific Endopeptidase from Flavobacterium sp.
lyophilized powder, ≥5.0 units/mg solid
Proline Specific Endopeptidase from Flavobacterium sp.
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
EndoProC, Endoprolylpeptidase, Endoproteinase Pro-C, PEPase, Prolyl Endopeptidase
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
50 units | Warenkorb auf Verfügbarkeit prüfen | CHF 556.00 |
Über diesen Artikel
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥5.0 units/mg solid
Biological source:
bacterial (Flavobacterium spp.)
CHF 556.00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
bacterial (Flavobacterium spp.)
form
lyophilized powder
specific activity
≥5.0 units/mg solid
mol wt
~78 kDa
shipped in
dry ice
storage temp.
−20°C
Application
Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research [1]. Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia [1].
Biochem/physiol Actions
An endopeptidase is a proteolytic peptidase that breaks peptide bonds of nonterminal amino acids. Product E1411 is specific for proline (Pro). The Prolyl endopeptidase (PEP) mechanism is thought to be an induced fit mechanism, where the native enzyme exists in a conformationally flexible opened state but is changed to a closed state upon substrate binding [1].
Physical form
White amorphous lyophilized powder
Other Notes
One unit causes the formation of one micromole of p-nitroaniline per minute under specific pH and temperature conditions.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥5.0 units/mg solid | specific activity ≥1,000 units/mg solid | specific activity ≥30 units/mg protein | specific activity ≥2 AU/mg protein |
| biological source bacterial (Flavobacterium spp.) | biological source bacterial (LYSOBACTER SP. BACTERIA M497-1) | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~78 kDa | mol wt 27 kDa | mol wt 28.93 kDa | mol wt - |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.