Biochimica et biophysica acta, 615(1), 237-245 (1980-09-09)
The extracts of granules isolated from bovine granulocytes show elastase- and chymotrypsin-like activities, as detected with specific synthetic substrates. Extraction of these enzymes depends upon salt concentration. In the course of the present studies a 21-fold purification of the elastase-like
Archives of microbiology, 153(1), 90-94 (1989-01-01)
The effect of the removal of signal peptides after cleavage of precursor molecules by the signal peptidase I was examined in an in vitro translocation system with Escherichia coli membrane vesicles. The translocation of periplasmic alkaline phosphatase precursors was significantly
The effect of low-molecular-weight inhibitors on the growth in vitro of a human pancreatic adenocarcinoma cell line.
A G Grant et al.
Biochemical Society transactions, 9(1), 134-134 (1981-02-01)
Increased activity of various proteases is observed in both human and experimental pancreatitis; however, the information on the effects of specific protease inhibitors on the disease is limited. In this study we show that a novel elastase inhibitor, guamerin-derived synthetic
Biochimica et biophysica acta, 661(2), 315-322 (1981-10-13)
The pyrimidine-3 gene of Neurospora crassa codes for a bifunctional enzyme catalysing the first two steps of the pyrimidine biosynthetic pathway. Difficulties have been experienced in purification due to the lability of the enzyme. The enzyme loses carbamoyl-phosphate synthetase (carbon-dioxide:
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