D4522

Desoxyribonukleinsäure aus Kalbsthymus

Type XV, Activated, lyophilized powder

• Synonym(e): DNA aus Kalbsthymus, Thymonukleinsäure
• CAS-Nummer: 91080-16-9
• UNSPSC Code: 41106310
• eCl@ss: 32160414
• EC Number: 293-507-3
• NACRES: NA.52
• MDL number: MFCD00151698
• Biological source: bovine pancreas
• Form: lyophilized powder
• Solubility: water: slightly soluble
• Storage temp.: −20°C

Eigenschaften

• biological source: bovine pancreas
• Quality Segment: 200
• grade: Molecular Biology
• type: Type XV
• form: lyophilized powder
• solubility: water: slightly soluble
• storage temp.: −20°C

Beschreibung

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Kälberthymus-DNA ist besonders nützlich als Substrat für DNA-Polymeraseassays, für die Amplifikation von sehr langen Fragmenten und als Träger-DNA für Ausfällungen.

Features and Benefits

• Nach 16 Std. Inkubation bei 37 °C wurde keine Nukleaseaktivität nachgewiesen
• Hochqualitative Template-DNA

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

One mg DNA is approx. 20 A₂₆₀ units.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves