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NACRES:
NB.51
UNSPSC Code:
41123100
Technischer Dienst
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Unterstützung erhaltentechnique(s)
dialysis: suitable
avg. diam.
20 mm , when full
avg. flat width
32 mm (1.3 in.)
capacity
~100 mL/ft
pore size
14000 MWCO, 99.99% retention
General description
Retains >99% of cytochrome c (M.W. 14,000) in solution over a 10-hour period.
Packaging
Supplied in rolls, dry
Preparation Note
Removal of glycerol included as a humectant can be accomplished by washing the tubing in running water for 3-4 hours. Removal of sulfur compounds can be accomplished by treating the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute. Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid. This tubing will retain most proteins of molecular weight 14,000 or greater.
Analysis Note
May contain glycerin and sulfur compounds in more than trace amounts; washing instructions are included.
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