C5863

Anti-Calcium Channel (β₄ Subunit) (Voltage Gated Ca²⁺ Channel) antibody produced in rabbit

~1 mg/mL, fractionated antiserum, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD04974685
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 5

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, rat, mouse
• concentration: ~1 mg/mL
• technique(s): western blot: 5-10 μg/mL using brain tissue lysate
• UniProt accession no.: O00305
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CACNB4(785) ; mouse ... Cacnb4(12298) ; rat ... Cacnb4(58942)

Beschreibung

General description

The gene CACNB4 (Calcium channel voltage-dependent subunit beta 4) is mapped to human chromosome 2q22-23. It is present at the membrane as well as the nucleus.

Immunogen

synthetic peptide derived from the rat β₄ calcium channel subunit conjugated to KLH.

Application

Anti-Calcium Channel (β-4 subunit) antibody produced in rabbit is suitable for western blot at a concentration of 5-10μg/mL using brain tissue lysate.
The antibody has been used for:

• western blotting of proteins extracted from different brain regions of the mouse
• western blotting of proteins isolated from primary culture of mouse cerebral cortical neurons

Biochem/physiol Actions

Specifically recognizes a splice variant of β₄ (55 kDa).

The CACNB4 (Calcium channel voltage-dependent subunit β 4) is an intracellular regulatory subunit of voltage-activated calcium channels that has significant impact on membrane expression and electrophysical characteristics of the channel. Calcium channel β subunits regulate voltage-dependent calcium currents through direct interaction with α1 subunits. Mutations in CACNB4 are associated with idiopathic generalized epilepsy and episodic ataxia. CACNB4 interacts with heterochromatin protein 1γ (HP1γ). The interaction attenuates the gene silencing activity by HP1.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids