C2230

Coelenterazine, native

solid

• Synonym(e): 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN
• Empirische Formel (Hill-System): C₂₆H₂₁N₃O₃
• CAS-Nummer: 55779-48-1
• Molekulargewicht: 423.46
• MDL-Nummer: MFCD00467176
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 24892477
• NACRES: NA.32

Eigenschaften

• Assay: ≥95% (TLC)
• Form: solid
• Löslichkeit: methanol and ethanol: soluble
• Lagertemp.: −20°C
• SMILES String: Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• InChIKey: YHIPILPTUVMWQT-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla, Oplophorus and Periphylla.

Anwendung

Coelenterazine, native was used:-

• For Aequorin assays
• For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells
• In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity

Biochem./physiol. Wirkung

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca²⁺ binds to the complex; used to measure Ca²⁺ concentration in cells with high sensitivity and large dynamic range.

Vorsicht

Protect from exposure to light

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)