B9183

Monoklonaler ANTI-HA-Antikörper

clone HA-7, purified from hybridoma cell culture

• Synonym(e): Anti-HA, Anti-Influenza-Hämagglutinin
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD00803873
• Conjugate: biotin conjugate
• Clone: HA-7, monoclonal
• Application: WB CL
• Citations: 7

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: biotin conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: buffered aqueous solution
• concentration: ~1 mg/mL
• technique(s): western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
• shipped in: dry ice
• storage temp.: 2-8°C

Beschreibung

General description

The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.

Immunogen

An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.

Application

Antibody suitable for immunoblotting.

Biochem/physiol Actions

Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable