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Merck

A4675

Sigma-Aldrich

Agar

Type E, suitable for plant cell culture

Synonym(e):

Agar-Agar

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About This Item

Lineare Formel:
(C12H18O9)n
CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21

Biologische Quelle

algae (Rhodophyceae)

Typ

Type E

Form

powder

Methode(n)

cell culture | plant: suitable

Übergangstemp.

transition temp 32-38 °C (1.5%)

Anwendung(en)

agriculture

Lagertemp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChIKey

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Allgemeine Beschreibung

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.
Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally, it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked ß-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Anwendung

Agar has been used as reagent for the following studies:
  • Plant cell culture studies.
  • As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
  • For the solidification of culture medium of cork oak somatic embryos.
  • to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants
Agar suspension culture may be used for the analysis of cell transformation of palyoma virus. It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.
Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Arsenic is a toxic metalloid and recognized carcinogen. Arsenate and arsenite are the most common arsenic species available for uptake by plants. As an inorganic phosphate (Pi) analog, arsenate is acquired by plant roots through endogenous Pi transport systems. Inside
Pere Puigderrajols et al.
Journal of experimental botany, 53(373), 1445-1452 (2002-05-22)
The timing and tissue localization of small heat shock proteins (sHSPs) during cork oak somatic embryo development was investigated under normal growing culture conditions and in response to stress. Western blot analyses using polyclonal antibodies raised against cork oak recombinant
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I MACPHERSON et al.
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B L Pike et al.
Journal of cellular physiology, 76(1), 77-84 (1970-08-01)
Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal (loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana
Uraguchi S, et al.
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