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A1602

Sigma-Aldrich

Amyloglucosidase aus Aspergillus niger

ammonium sulfate suspension, ≥40 units/mg protein

Synonym(e):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS-Nummer:
9032-08-0
EC-Nummer:
3.2.1.3 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00081350
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

ammonium sulfate suspension

Spezifische Aktivität

≥40 units/mg protein

Versandbedingung

wet ice

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Anwendung

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .
Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Biochem./physiol. Wirkung

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Einheitendefinition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physikalische Form

Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0

Sonstige Hinweise

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae
Teets NM, et al.
The Journal of Experimental Biology, 214(5), 806-814 (2011)
Jane Usher et al.
Applied and environmental microbiology, 75(13), 4573-4579 (2009-05-12)
Yeasts used in the production of lagers contain complex allopolyploid genomes, resulting from the fusion of two different yeast species closely related to Saccharomyces cerevisiae and Saccharomyces bayanus. Recombination between the homoeologous chromosomes has generated a number of hybrid chromosomes.
Christina Pedersen et al.
PloS one, 13(3), e0194468-e0194468 (2018-03-21)
Insulin is a vital part of diabetes treatment, whereas glucagon is primarily used to treat insulin-induced hypoglycemia. However, glucagon is suggested to have a central role in the regulation of body weight, which would be beneficial for diabetic patients. Since
A method for routine measurements of total sugar and starch content in woody plant tissues
Chow PS and Landhausser SM
Tree physiology, 24(10), 1129-1136 (2004)
Hugh S Winwood-Smith et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)
Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores
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Artikel

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protokolle

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

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