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A0410

Sigma-Aldrich

Aminoallyl-dUTP Natriumsalz

≥85%, lyophilized powder

Synonym(e):

5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

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About This Item

Empirische Formel (Hill-System):
C12H20N3O14P3 · xNa+
CAS-Nummer:
936327-10-5
Molekulargewicht:
523.22 (free acid basis)
UNSPSC-Code:
41106305
NACRES:
NA.51

Qualitätsniveau

200

Assay

≥85%

Form

lyophilized powder

Versandbedingung

dry ice

Lagertemp.

−70°C

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Anwendung

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
  • for the generation of chromatin immunoprecipitation (ChIP) probes
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem./physiol. Wirkung

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
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