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Merck

07985

6-Aminofluoreszein

BioReagent, suitable for fluorescence, ~95% (HPLC)

Synonym(e):

Fluoresceinamin Isomer II

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1 G

CHF 202.00

5 G

CHF 399.50

CHF 202.00


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Über diesen Artikel

Empirische Formel (Hill-System):
C20H13NO5
CAS-Nummer:
Molekulargewicht:
347.32
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
257-334-7
MDL number:
Beilstein/REAXYS Number:
51708

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Unterstützung erhalten

product line

BioReagent

Quality Level

assay

~95% (HPLC)

form

powder

impurities

≤5% 5-aminofluorescein

mp

285 °C (dec.) (lit.)

solubility

DMSO: soluble, acetone: soluble, methanol: soluble

fluorescence

λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0

suitability

suitable for fluorescence

SMILES string

Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2

InChI key

YOAWSYSKQHLFPM-UHFFFAOYSA-N

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Dieser Artikel
20163434321-M408948
suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0

fluorescence

-

fluorescence

-

fluorescence

-

form

powder

form

powder

form

solid

form

liquid

solubility

DMSO: soluble, methanol: soluble, acetone: soluble

solubility

methanol: 1 mg/mL, clear, yellow to very dark yellow-orange

solubility

DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble

solubility

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

assay

~95% (HPLC)

assay

-

assay

≥95% (TLC)

assay

95%

Application

6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.

Other Notes

Nicht zu verwechseln mit Fluorescamin.

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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K C Ingham et al.
Biochimica et biophysica acta, 670(2), 181-189 (1981-09-29)
A method for covalent attachment of a fluorescent molecule to the carbohydrate moieties of glycoproteins is described. The glycoproteins were oxidized with periodate under mild conditions selective for sialic acid (Van Lenten, L. and Ashwell, G. (1971) J. Biol. Chem.
I Climent et al.
Analytical biochemistry, 182(2), 226-232 (1989-11-01)
Oxidative modification of proteins is implicated in a number of physiologic and pathologic processes. Metal-catalyzed oxidative modification usually causes inactivation of enzymes and the appearance of carbonyl groups in amino acid side chains of the protein. We describe use of
Miju Kim et al.
ACS nano, 9(8), 8269-8278 (2015-07-15)
Polyelectrolyte microcapsules represent versatile stimuli-responsive structures that enable the encapsulation, protection, and release of active agents. Their conventional preparation methods, however, tend to be time-consuming, yield low encapsulation efficiency, and seldom allow for the dual incorporation of hydrophilic and hydrophobic
Thapakorn Tree-Udom et al.
ACS applied materials & interfaces, 7(43), 23993-24000 (2015-10-16)
Although computer simulation and cell culture experiments have shown that elongated spherical particles can be taken up into cells more efficiently than spherical particles, experimental investigation on effects of these different shapes over the particle-membrane association has never been reported.
Thomas Tigges et al.
Small (Weinheim an der Bergstrasse, Germany), 11(35), 4540-4548 (2015-06-06)
Patchy particles are next generation colloidal building blocks for self-assembly and find further use as (bio) sensors. Progress in this direction crucially depends on developing straightforward preparation pathways able to provide patchy particles with highest uniformity and integrating precise, orthogonal

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Global Trade Item Number

SKUGTIN
D193801-100G04061831835724
07985-5G04061832531328
07985-1G04061826660836

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