MAB343

Anti-Amyloid Precursor Protein (APP) Antibody

CHEMICON^®, mouse monoclonal, 2.F2.19B4

• Synonym(e): APP, Jonas clone
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 2.F2.19B4, monoclonal
• Species reactivity: rat, human
• Application: IHC, WB
• Citations: 35

Eigenschaften

• Produktname: Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4, ascites fluid, clone 2.F2.19B4, Chemicon^®
• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• clone: 2.F2.19B4, monoclonal
• species reactivity: rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000484.2, NM_201413.1, NM_201414.1
• UniProt accession no.: P05067
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Beschreibung

Immunogen

Carboxyl fragment of APP 643-695 / Jonas.

Epitope: APP 643-695 C-terminal fragment

Application

Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.

Western blot: 10 μg/mL

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB343

IMMUNOHISTOCHEMISTRY

1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).

2) Wash sections 3 times in distilled water.

3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.

4) Wash sections for 5 min in TBS.

5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.

6) Wash sections 3 times in TBS for 5 min..

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in TBS.

9) Embed sections and examine.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.

Biochem/physiol Actions

Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html

Physical form

Liquid.

Unpurified

Preparation Note

Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable