CC127

Caspase 2, recombinant human active

• eCl@ss: 32160405
• UNSPSC Code: 12352200

Eigenschaften

• biological source: human
• form: solid
• specific activity: 25 units
• manufacturer/tradename: Chemicon^®
• technique(s): activity assay: suitable
• NCBI accession no.: NM_032982.2, NM_032983.2
• UniProt accession no.: P42575
• shipped in: dry ice
• Gene Information: human ... CASP2(835)

Beschreibung

General description

Caspase-2 (also known as ICH-1, NEDD-2) is a member of the interleukin-1beta converting enzyme (ICE) family of cystein proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19 kDa) and small (12 kDa) subunits which associate to form the active enzyme. The active recombinant human caspase-2 was expressed in E. coli. The expressed caspase-2 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-2 preferentially cleaves the substrates consisting of consensus sequence VDVAD (e.g., VDVAD-AFC and VDVAD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates.Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user.

Physical form

Partially purified recombinant caspase 2 with a full length HIS-6-tag. Approximately 10-20% caspase 2 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity. Lyophilized. Reconstitute in 25 μL PBS containing 15% glycerol.

Preparation Note

Maintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately.

Analysis Note

Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 2 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity.

Other Notes

Specific Activity: Where one unit of the recombinant caspase-2 is the enzyme activity that cleaves 1 nmol of the caspase substrate VDVAD-pNA (pNA: pnitroanaline) per hour at 37oC in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable