CBL496

Anti-CD34 Class II Antibody, clone QBEND/10

clone QBEND/10, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: QBEND/10, monoclonal
• Species reactivity: monkey, human
• Application: FACS, IHC (p), IP
• Citations: 10

Eigenschaften

• biological source: mouse
• Quality Segment: 100, 300
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: QBEND/10, monoclonal
• species reactivity: monkey, human
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
• isotype: IgG1
• NCBI accession no.: NM_001025109.1, NM_001773.2
• UniProt accession no.: P28906
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CD34(947)

Beschreibung

Immunogen

Human endothelial vesicles

Application

Anti-CD34 Antibody, Class II, clone QBEND/10 is an antibody against CD34 Class II for use in FC, IP, IH, IH(P).

Identification of haemopoietic progenitor cells

Studies into the control mechanisms of early haemopoiesis

Purification of haemopoietic cells from, for example, FACS

The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts

Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.

Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.

KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.

FUSION PARTNER: NSO myeloma cell line

Physical form

Format: Purified

The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Analysis Note

Tested

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.