CA1026

Anti-Wilm′s Tumor Protein Rabbit pAb

liquid, Calbiochem^®

• Synonym(e): Anti-WT-1
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse, human, rat
• Application: —
• Citations: 14

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: mouse, human, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• isotype: IgG
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... WT1(7490)

Beschreibung

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~47-55 kDa Wilm′s tumor protein.

Recognizes the ~47-55 kDa Wilm′s tumor protein in Wilm′s tumor tissue.

This Anti-Wilm′s Tumor Protein Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections, Immunoprecipitation for the detection of Wilm′s Tumor Protein.

Immunogen

Human

a synthetic peptide corresponding to amino acids from the C-terminus of human Wilm′s tumor protein

Application

Immunoblotting (see comments)
Paraffin Sections (1:100, heat pre-treatment required)
Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM PBS, 0.2% BSA, pH 7.6.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage.

Analysis Note

Positive Control
Wilm′s tumor tissue

Other Notes

Huff, V. 1998. Am. J. Med. Genet.79, 260.

Staining of formalin-fixed, paraffin-embedded tissues requires antigen retrieval by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at room temp for 20 min. This antibody is also reported to work for immunoblotting and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable