T6778
Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate
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About This Item
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biological source
goat
Quality Level
conjugate
TRITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
contains
≥15 mM sodium azide as preservative
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:400
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
storage temp.
−20°C
target post-translational modification
unmodified
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General description
Binds all rabbit Igs.
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.
Application
Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.
Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.[1]
Biochem/physiol Actions
IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Other Notes
Antibody adsorbed with human IgG.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4.
Preparation Note
Adsorbed to reduce background staining with human samples.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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