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I9392

Sigma-Aldrich

3-Methylbutanol

for molecular biology, BioReagent, ≥98.5%

Synonym(s):

3-Methyl-1-butanol, Isoamyl alcohol, Isopentyl alcohol

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About This Item

Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
123-51-3
Molecular Weight:
88.15
Beilstein:
1718835
EC Number:
204-633-5
MDL number:
MFCD00002934
UNSPSC Code:
12352200
PubChem Substance ID:
24896157
NACRES:
NA.52

grade

for molecular biology

product line

BioReagent

Assay

≥98.5%

form

liquid

technique(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.
3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.

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Description
Pricing

Signal Word

Danger

Hazard Statements

H226,H315,H318,H332,H335

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

110.3 °F - closed cup

Flash Point(C)

43.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
Niels O Verhulst et al.
Malaria journal, 10, 28-28 (2011-02-10)
Anopheles gambiae sensu stricto is considered to be highly anthropophilic and volatiles of human origin provide essential cues during its host-seeking behaviour. A synthetic blend of three human-derived volatiles, ammonia, lactic acid and tetradecanoic acid, attracts A. gambiae. In addition
Luca Bianco et al.
PloS one, 9(10), e110377-e110377 (2014-10-11)
High-density SNP arrays for genome-wide assessment of allelic variation have made high resolution genetic characterization of crop germplasm feasible. A medium density array for apple, the IRSC 8K SNP array, has been successfully developed and used for screens of bi-parental
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