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I6385

Sigma-Aldrich

Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse

clone MB-11, ascites fluid

Synonym(s):

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Anti Human IgM Antibody - Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse, Anti Human Igm Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

Quality Level

300

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

MB-11, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Human serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.
Mouse monoclonal clone MB-11 anti-Human IgM (μ-chain specific) antibody is specific for the μ-chain of human IgM as determined by an ELISA. It does not bind human IgG, IgA, or light chains.

Application

Mouse monoclonal clone MB-11 anti-Human IgM (μ-chain specific) antibody may be used to identify and quantitate the level of human IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques. Elisa assays were performed using alkaline phosphatase conjugated anti-human IgM to detect IgMs in phage supernatants and human sera at 1:1000 dilution.[1]

Biochem/physiol Actions

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000350038
0000222573
0000221824
0000218333
0000210170

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Menke de Smit et al.
Arthritis research & therapy, 14(5), R222-R222 (2012-10-19)
The association between rheumatoid arthritis (RA) and periodontitis is suggested to be linked to the periodontal pathogen Porphyromonas gingivalis. Colonization of P. gingivalis in the oral cavity of RA patients has been scarcely considered. To further explore whether the association
Emerging functions of natural IgM and its Fc receptor FCMR in immune homeostasis.
Wang H, et al.
Frontiers in Immunology, 7(11), 99-99 (2016)
M Mecchia et al.
Journal of immunology (Baltimore, Md. : 1950), 157(8), 3727-3736 (1996-10-15)
Type II mixed cryoglobulinemia (CryoII) is an autoimmune disorder frequently associated to human hepatitis C virus (HCV) infection and characterized by the presence of cold-insoluble immunocomplexes containing IgM with rheumatoid activity. To identify disease-related epitopes, we screened a phage-displayed random
C Oeuvray et al.
Infection and immunity, 68(5), 2617-2620 (2000-04-18)
The goal of this study was to analyze antibody responses to Plasmodium falciparum glutamate-rich protein (GLURP) using clinical data and plasma samples obtained from villagers of Dielmo, Senegal. This molecule was chosen because it is targeted by human antibodies which
Protective roles of natural IgM antibodies
Gronwall C, et al.
Frontiers in Immunology, 3, 66-66 (2012)
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Questions

  1. I am inquiring about the quantity of total protein in the ascites fluid.

    1 answer

    1. The total protein varies from lot to lot and is reported in the product Certificate of Analysis. The amount ranges from 15-55 mg/mL. Please see the link below to review a sample or lot specific certificate:
      https://www.sigmaaldrich.com/product/sigma/i6385#product-documentation

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/295/466/i6385dat.pdf

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