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H0878

Sigma-Aldrich

Heparin lithium salt from porcine intestinal mucosa

≥150 USP units/mg

Synonym(s):

Lithium acid heparin, Lithium heparinate

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About This Item

CAS Number:
9045-22-1
MDL number:
MFCD00131308
UNSPSC Code:
12352201
NACRES:
NA.25
Pricing and availability is not currently available.

biological source

Porcine intestinal mucosa

Quality Level

200

form

powder

specific activity

≥150 USP units/mg

color

off-white

solubility

water: 40 mg/mL, clear, colorless to faint yellow or tan

cation traces

Li: 3-4%

application(s)

diagnostic assay manufacturing
life science and biopharma
plasma purification
sample preparation

storage temp.

room temp

SMILES string

[S](=O)(=O)(NC1C(OC(C(C1O)OC2OC(C(C(C2O[S](=O)(=O)O)O)OC)C(=O)O)CO[S](=O)(=O)O)OC)O

InChI

1S/C14H25NO20S3/c1-29-9-7(17)10(35-38(26,27)28)14(34-11(9)12(18)19)33-8-4(3-31-37(23,24)25)32-13(30-2)5(6(8)16)15-36(20,21)22/h4-11,13-17H,3H2,1-2H3,(H,18,19)(H,20,21,22)(H,23,24,25)(H,26,27,28)

InChI key

HXSDFQWQRCUQHF-UHFFFAOYSA-N

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General description

Heparin lithium is a highly sulfated polysaccharide that belongs to the family of glycosaminoglycans with remarkable significance in the areas of cell culture, cancer biology, biochemical, and drug discovery research. Due to its interactions with proteins and cells, it is used to study molecular and cellular processes, tumor modeling, investigate binding interactions, and as a cell culture supplement, to maintain cell viability.

Application

Heparin lithium was used:
  • To validate photometric measurements in cell surface charge determination[1]
  • As an anti-coagulant for laboratory studies[2]
  • To study hypothermal stress on lactate metabolism[3]
  • To study the effects of anticoagulants in direct membrane feeding assays[2]
  • For 2D and 3D vitro cell culture in glioblastoma cell models[4]
  • To prevent clot formation and tissue damage during mechanical probing of cardiac pacemaker cell embryonic patterning, a study that contributes to the broader area of cardiac tissue mechanics research and developmental biology[5]

Features and Benefits

  • Heparin with specific activity of ≥150 USP units/mg
  • Versatile and adaptable for a wide variety of laboratory and research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000428134
0000428134
0000420690
0000420690
0000416756

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Chia-Hao Chang et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 248, 110744-110744 (2020-06-23)
The milkfish Chanos chanos, an economically important cultured marine species in Southeast Asia, exhibits stenothermal and euryhaline characteristics and huge mortality usually occurs during extreme cold weather in winter. Under conditions beyond optimal temperatures, ectothermic species experience an increase in
The effect of anticoagulants in blood collection tubes on Plasmodium falciparum transmission in direct membrane feeding assays
Wouter G et al.
Transactions of the Royal Society of Tropical Medicine and Hygiene, 116, 187- 189 (2022)
Determination of cell surface charge by photometric titration
Thethi K et al
Journal of Biochemical and Biophysical Methods, 34, 137-145 (1997)
R B Shartau et al.
The Journal of experimental biology, 223(Pt 7) (2020-03-05)
Acute (<96 h) exposure to elevated environmental CO2 (hypercarbia) induces a pH disturbance in fishes that is often compensated by concurrent recovery of intracellular and extracellular pH (pHi and pHe, respectively; coupled pH regulation). However, coupled pH regulation may be limited
Beatriz Giesen et al.
Pharmaceutics, 13(2) (2021-03-07)
Gold nanoparticles (Au NPs) are studied as delivery systems to enhance the effect of the glutaminase1 inhibitor CB839, a promising drug candidate already in clinical trials for tumor treatments. Au NPs were synthesized using a bottom-up approach and covered with
View All Related Papers

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Questions

1–7 of 7 Questions  

  1. Could you provide the procedure for making a 20 IU/mL solution in saline using the H0878-100KU? e.g.) 1) Add 5 Liter of saline in H0878-100KU 1 bottle (volume/weight, %) 2) Vortex thoroughly 3) Filtration (0.2 um pore size) 4) Keep it at 2-4'C

    1 answer

    1. In order to prepare the solution at 20 IU/mL, the activity would be required. The activity of this product is lot specific and reported on the Certificate of Analysis. The minimum activity for any lot would be 150 units/mg. See the link below to review a sample or access the Certificate of a specific lot:
      https://www.sigmaaldrich.com/product/sigma/h0878#product-documentation

      This material is soluble at 40 mg/mL in water. The same rate would be expected in saline, but rates may vary slightly. Stirring is recommended, however vigorous vortexing is not typically required.
      Please see the link below to review the product data sheet which includes information on solubility and solution stability:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/743/456/h0878pis.pdf

      Helpful?

  2. How much heparin is needed to prevent coagulation in whole blood?

    1 answer

    1. It is suggested to use 15 to 30 units per mL of blood.

      Helpful?

  3. There is no expiration date on our heparin lithium salt. The lot # is 76H1236. Is this product usable?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of roughly 2 years after the quality release date. The lot number given (76H1236) was released in 1996. The contents may demonstrate some activity if the bottle is unopened, however it is unlikely that it would meet specifications. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the solution stability of Product H0878, Heparin lithium salt from porcine intestinal mucosa?

    1 answer

    1. Heparin is a polysaccharide, which has excellent aqueous solution stability (up to 2 years at 2-8 °C) provided the solutions have been sterilized by filtration through a 0.2 μm membrane.  More solution stability information can be on the product information sheet (under Documents, above).

      Helpful?

  6. How can Product H0878, Heparin lithium salt from porcine intestinal mucosa be reconstituted?

    1 answer

    1. The solubility of heparin is 40 mg/mL in water.

      Helpful?

  7. What is the potency of Product H0878, Heparin lithium salt from porcine intestinal mucosa?

    1 answer

    1. The specification for potency is ? 150 USP units/mg.  The lot specific potency can be found on the Certificate of Analysis, which can be accessed on the Product Detail page.

      Helpful?

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