G3512
Glycerol Dehydrogenase from Cellulomonas sp.
lyophilized powder, ≥50 units/mg protein
Synonym(s):
Glycerol:NAD+ 2-oxidoreductase
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About This Item
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
bacterial (Cellulomonas spp.)
Quality Level
form
lyophilized powder
specific activity
≥50 units/mg protein
mol wt
~390 kDa
composition
Protein, ≥60% biuret
storage temp.
−20°C
General description
Application
Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method.[3] It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.[4][5]
This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.[6]
Biochem/physiol Actions
Physical properties
Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni++, Cu++, Zn++, Cd++)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH+4 and Rb+.
Michaelis constants : 1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni++, Cu++, Zn++, Cd++)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH+4 and Rb+.
Unit Definition
One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.
Physical form
Lyophilized powder containing bovine serum albumin
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Glycerol dehydrogenase: structure, specificity, and mechanism of a family III polyol dehydrogenase
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An improved glycerol biosensor with an Au-FeS-NAD-glycerol-dehydrogenase anode
Mahadevan A and Fernando S
Biosensors And Bioelectronics, 92, 417-424 (2017)
Sequential injection kinetic flow assay for monitoring glycerol in a sugar fermentation process by Saccharomyces cerevisiae
Dominguez KBH, et al.
Applied Biochemistry and Biotechnology, 160(6), 1664-1673 (2010)
Further characterization of glycerol dehydrogenase from Cellulomonas sp. NT3060
Nishise H, et al.
Agricultural and Biological Chemistry, 48(6), 1603-1609 (1984)
Simultaneous detection of ethanol, glucose and glycerol in wines using pyrroloquinoline quinone-dependent dehydrogenases based biosensors
Niculescu M, et al.
Food Chemistry, 82(3), 481-489 (2003)
Protocols
Assay Procedure for Glycerol Dehydrogenase
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