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F6140

Sigma-Aldrich

Anti-Fibronectin Antibody

mouse monoclonal, FN-3E2

Synonym(s):

Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF

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About This Item

MDL number:
MFCD00164562
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

Product Name

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse, clone FN-3E2, ascites fluid

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FN-3E2, monoclonal

mol wt

antigen 240 kDa

contains

15 mM sodium azide

species reactivity

mouse, human, chicken

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 1:400 using cultured chicken fibroblasts
radioimmunoassay: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

P02751

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

Related Categories

General description

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Specificity

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:
  • immunoblot
  • immunofluorescent microscopy
  • immunocytochemistry

Biochem/physiol Actions

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .
Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000401255
0000367296
0000401255
0000367296
0000284777

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Fibronectin in cell adhesion and migration via N-glycosylation
Hsiao CT, et al.
Oncotarget, 8(41), 70653-70653 (2017)
Critical role of the cAMP-PKA pathway in hyperglycemia-induced epigenetic activation of fibrogenic program in the kidney
Deb DK, et al.
Faseb Journal, 31 (2017)
S Stenman et al.
Acta medica Scandinavica. Supplementum, 642, 165-170 (1980-01-01)
Fibronectin is a polymorphic glycoprotein of plasma, other body fluids and connective tissue, and it occurs in an insoluble and a soluble form. Insoluble fibronectin is found associated with basement membranes and in loose connective tissue matrix as well as
A Vaheri
Schweizerische medizinische Wochenschrift, 110(40), 1437-1440 (1980-10-04)
Fibronectin is a high molecular weight glycoprotein which is found in a soluble form in plasma (cold-insoluble globulin) and other body fluids and in an insoluble form in connective tissues and associated with basement membranes. Soluble fibronectin interacts with denatured
Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment
McLane JS, et sl.
Biophysical Journal, 109 (2015)
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Questions

  1. does this anti-cellular fibronectin target EDA slice in cell-secreted fibronectin?

    1 answer

    1. This product is not tested specifically for detection of the EDA region, however these papers claim that this antibody reacts with the alternatively spliced extra domain (EIIIA) of cellular fibronectin:
      1. Barnes, J.V. et al., J. Histochem. Cytochem., 47, 533-543 (1999).
      2. Barnes, V.L. et al., J. Histochem. Cytochem., 47, 787-797 (1999).

      Alternatively, please review product MAB1940, which is specific for the extra domain of cellular fibronectin (EDA sequence) and thus recognizes only the cellular fibronectin. See below for a link to the product page:
      https://www.sigmaaldrich.com/product/mm/mab1940

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