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F4680

Sigma-Aldrich

Fluoromount™ Aqueous Mounting Medium

liquid

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.

Product Name

Fluoromount™ Aqueous Mounting Medium, for use with fluorescent dye-stained tissues

grade

for fluorescence

Quality Level

200

form

liquid

technique(s)

microbe id | staining: suitable

color

clear

suitability

suitable for immunohistochemistry

application(s)

diagnostic assay manufacturing
hematology
histology

Related Categories

General description

Fluoromount is an aqueous based mounting medium,similar to Gel Mountä, for use with coverslips to preserve tissues stained with fluorescent dyes. Thisproduct is compatible with FITC, phycoerythrin, phycocyanin, and allophycocyanin labeling. It can alsobe used with other fluorescent markers such as TexasRedÒand rhodamine. The medium after use can easily be removed by soaking the slides in deionized water.

Application

Fluoromount™ Aqueous Mounting Medium has been used for mounting:
  • coverslips on slides for immunofluorescence microscopic examination of PC12 and Hela cells[1]
  • hippocampus neurons cells for confocal microscope studies[2]
  • brain sections for immunofluorescence staining[3]

Legal Information

Fluoromount is a trademark of Diagnostic BioSystems, Inc.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000410298
0000405984
0000403238
0000403315
0000403313

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David Fast et al.
mBio, 9(4) (2018-08-02)
Adult Drosophila melanogaster raised in the absence of symbiotic bacteria have fewer intestinal stem cell divisions and a longer life span than their conventionally reared counterparts. However, we do not know if increased stem cell divisions are essential for symbiont-dependent
Biophysics of microchannel-enabled neuron-electrode interfaces
Wang L, et al.
Journal of Neural Engineering, 9(2), 026010-026010 (2012)
Kyle J Martin et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(51), 14769-14774 (2016-12-09)
Teeth and denticles belong to a specialized class of mineralizing epithelial appendages called odontodes. Although homology of oral teeth in jawed vertebrates is well supported, the evolutionary origin of teeth and their relationship with other odontode types is less clear.
The role of chaperone-mediated autophagy in huntingtin degradation
Qi L, et al.
Testing, 7(10), e46834-e46834 (2012)
Sukant Garg et al.
Marine drugs, 17(6) (2019-06-15)
Fucoxanthin is commonly found in marine organisms; however, to date, it has been one of the scarcely explored natural compounds. We investigated its activities in human cancer cell culture-based viability, migration, and molecular assays, and found that it possesses strong
View All Related Papers

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Questions

1–10 of 10 Questions  

  1. What are some examples of aqueous mounting media without glycerol?

    1 answer

    1. For use with fluorescence:

      1. F4680 Fluoromount
      2. F6182 Fluoroshield

      Aqueous Mounting media with no glycerol and antifade agents:

      1. 10981 Polyvinyl alcohol with DABCO
      2. F6937 Fluoroshield with DABCO
      3. F6812 Fluoroshield with PPD (not suitable for a select number of fluorescent dyes)

      Aqueous Mounting Media with no glycerol and another dye:

      1. F6057 Fluoroshield with DAPI
      2. F5932 Fluoroshield with PI

      Aqueous Mounting Media suitable for use with light microscopy:

      1.GG1 Glycerol gelatin
      2.C9368 CC/Mount

      Helpful?

  2. Is F4680 suitable for use as a mounting medium for AEC red IHC mounting?

    1 answer

    1. Fluoromount is not intended for use with bright field microscopy or for coverslipping slides stained with AEC. Most fluorescent mounting media are somewhat alkaline, with a pH around 9, while AEC is typically formulated with an acetate buffer around pH 5.2. The difference in pH levels suggests that the AEC may fade quickly, especially if the slides are counterstained with a nuclear counterstain such as Mayer's Hematoxylin, which would also likely disappear rapidly.

      Helpful?

  3. Does this (Fluoromount F4680) contain DAPI?

    1 answer

    1. This product does not contain DAPI. Should DAPI be preferred, see product F6057.

      F4680 Fluoromount datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/692/371/f4680pis.pdf

      F6057 Fluoroshield with DAPI product page:
      https://www.sigmaaldrich.com/product/sigma/f6057

      Helpful?

  4. Does Fluoromount (Product No. F4680) remain a liquid or solidify?

    1 answer

    1. Fluoromount will solidify as it dries. This typically takes 30-40 minutes.

      Helpful?

  5. How do you remove the coverslip after using Fluoromount (Product No. F4680)?

    1 answer

    1. Place the slide in a beaker full of deionized water on a magnetic stirrer. Gently stir the slide for a few hours to overnight for complete removal of dry Fluoromount.

      Helpful?

  6. Does Fluoromount mounting media (Product No. F4680) contain glycerol?

    1 answer

    1. Fluoromount does not contain glycerol. The exact formulation is considered proprietary.

      Helpful?

  7. How does Product F4680, Fluoromount Mounting Medium, compare to GelMount (Product No. G0918) which has been deleted?

    1 answer

    1. Product No. F4608, Fluoromount is essential the same as GelMount. GelMount was deleted by the supplier. Fluoromount is being made by another supplier with the same formulation.

      Helpful?

  8. Does Fluoromount mounting media (Product No. F4680) contain an antifade reagent?

    1 answer

    1. Fluoromount does not contain an antifade reagent.  If you need an antifade mounting media, check Fluoroshield (Product Nos. F6182, F6057 and F5932) or PVA with antifade (Product Nos. 10981 and 10979).

      Helpful?

  9. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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