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D5941

Sigma-Aldrich

Drabkin′s reagent

for the determination of hemoglobin

Synonym(s):

Hemoglobin Quantitation Reagent

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About This Item

UNSPSC Code:
12352402
NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

200

form

liquid

General description

Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components.[1] Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin. The cyanmethemoglobin levels is estimated at 530nm. It is a reliable method and can measure all haemoglobin forms except sulphaemoglobin.[2]

Application

Drabkin′s reagent has been used for the quantitative colorimetric determination of blood haemoglobin in cerebral tissue to assess cerebral haemorrhage,[3] in red blood cells to detect hemolysis[4] and in contralateral and ipsilateral brain tissue homogenate.[5]

Components

Contains sodium bicarbonate, potassium ferricyanide, and potassium cyanide.

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Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2

Target Organs

Thyroid

Supplementary Hazards

EUH032

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000356469
0000334454
0000291849
0000356469
0000334454

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Toxic effects of silver nanoparticles and nanowires on erythrocyte rheology
Kim MJ and Shin S
Food And Chemical Toxicology, 67(6), 80-86 (2014)
Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods.
Balasubramaniam P and Malathi A
Journal of Postgraduate Medicine, 38(1), 8-8 (1992)
Drabkin, D.L., Austin, J.H.
The Journal of Biological Chemistry, 112, 51-51 (1935)
Kang Nian Yap et al.
The Journal of experimental biology, 220(Pt 22), 4282-4291 (2017-09-28)
Foraging to obtain food, either for self-maintenance or at presumably elevated rates to provide for offspring, is thought to be an energetically demanding activity but one that is essential for fitness (higher reproductive success and survival). Nevertheless, the physiological mechanisms
Allison Cornell et al.
The Journal of experimental biology, 220(Pt 21), 4060-4067 (2017-09-17)
In avian species, little is known about the development of physiological traits in the days preceding fledging, a critical life history transition marked by a high mortality rate. Developmental trajectory during this period may be flexible based on ecological context
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Questions

1–10 of 17 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How does freezing affect the stability and shelf life of Drabkin's Solution? Would freezing help maintain its efficacy for a longer duration?

    1 answer

    1. The stability of the Drabkin's solution when stored frozen has not been evaluated. The prepared solution is stable for at least 6 months at room temperature when stored protected from light. While it is possible that frozen storage would not impact the quality, this cannot be guaranteed. Please see the link below to review a publication that may be helpful.
      'The effect of freezing on the potassium ferricyanide-potassium cyanide reagent used in the cyanmethemoglobin procedure for hemoglobin determination'
      https://www.sciencedirect.com/science/article/abs/pii/0009898164901251

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  4. Can the concentration be calculated using Beer's law with the extinction coefficient of cyanmethemoglobin?

    1 answer

    1. The Molar Extinction coefficient is necessary to determine the cyanmethemoglobin concentration according to the Beer Lambert's law. The extinction coefficient has been reported in several publications. Please see the link below to review an example:
      https://academic.oup.com/ajcp/article-abstract/55/2/135/1769842

      Using the published coefficient at a specific wavelength, Beer's law may be used to calculate the concentration of cyanmethemoglobin in a given sample, based on its absorbance at that wavelength and the path length of the cuvette. The Drabkin's Reagent will allow for the determination of the total hemoglobin concentration from a linear calibration curve and the instructions for use for this product. Please see the link below to review:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf

      Helpful?

  5. Is the hemoglobin (standard) provided suitable for use with Drabkin's Reagent (D5941)?

    1 answer

    1. H2500 is recommended for use with Drabkin's reagent, as most mammalian hemoglobins are expected to yield similar absorbances with Drabkin reagent D5941, suggesting that porcine, rat, bovine, or human hemoglobin standards may also work. Additionally, a specific Porcine hemoglobin product, H4131, is available.

      Helpful?

  6. Can the Drabkins reagent detect hemoglobin down to 0.05 mg/dL

    1 answer

    1. The Drabkins reagent lacks the sensitivity to detect hemoglobin at levels as low as 0.05 mg/dl, with the approximate sensitivity being in the 3 to 4 mg/dl range.

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  7. How to store the configured drabkin reagent and how long it can be stored

    1 answer

    1. Store the Drabkin’s Reagent at room temperature protected from light. The powdered Drabkin’s Reagent is stable for at least 2 years. The prepared Drabkin’s Solution is stable for at least 6 months when stored at room temperature and protected from light (amber bottle).

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  8. what is the dilution ration that you are using for drabkin's reagent to distilled water? 1:40? Since you can't tell me what the volume is for one vial of the reagent

    1 answer

    1. A vial of Drabkin's reagent D5941 contains 1.25 g of the powder mixture as follows:

      Sodium bicarbonate, CAS # 144-55-8 || 80% (w / w) or 1000 mg per vial
      Potassium ferricyanide, CAS # 13746-66-2 || 16% (w / w) or 200 mg per vial
      Potassium cyanide, CAS # 151-50-8 || 4% (w / w) or 50 mg per vial

      The vial should be reconstituted with 1,000 mL of water and 0.5 mL of 30 % Brij L23 Solution.

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  9. How many mL are in one vial of the drabkin's reagent?

    1 answer

    1. This product is a prepared concentrate. Each vial of reagent is sufficient to prepare 1 L of Drabkin's solution. The volume of the concentrate is irrelevant, and thus not reported.
      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf

      Helpful?

  10. How much of the derbkin solution do I add for 10 mL of blood?

    1 answer

    1. Please access the Product Datasheet below. It indicates adding 20 uL of blood to a tube containing 5 mL of the Drabkin's solution (prepared according to linked protocol). Working with a volume of blood as large as 10 mL does not seem applicable to this product:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/139/718/d5941dat.pdf

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1–10 of 17 Questions  

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