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Key Documents

LC-2/ad

94072247, human lung, Epithelial

Synonym(s):

LC-2/ad Cells

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About This Item

UNSPSC Code:
41106514

product name

LC-2/ad, 94072247

biological source

human lung

growth mode

Adherent

karyotype

Modal No 53-56

morphology

Epithelial

products

alpha-1-anti-trypsin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human lung adenocarcinoma

Cell Line Description

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

Application

LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.

Culture Medium

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/ EDTA ; 5%CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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Articles

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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