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C-39420

Sigma-Aldrich

Melanocyte Growth Medium M2

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonym(s):

Recommended with C-24400 or C-24405

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

Pricing and availability is not currently available.

form

aqueous solution

packaging

pkg of 1 vial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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This Item
HPA021054HPA049153HPA047415
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:500-1:1000

technique(s)

immunohistochemistry: 1:50- 1:200

UniProt accession no.

O60704

UniProt accession no.

O60704

UniProt accession no.

Q15661

UniProt accession no.

O60507

General description

Melanocyte Growth Medium M2 SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-24400 or C-24405

Application

Melanocyte Growth Medium M2 is a serum-free and PMA (Phorbol Myristate Acetate)-free Medium that was developed for the optimized in vitro cultivation of human melanocytes. It is also suitable for human pigment epithelial cells, neuronal cells, and melanoma cells. The medium is optimized for primary human cells, but can also be used for equine, porcine, mouse, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin, cultured in M2 MediumNormal Human Epidermal Melanocytes (NHEM), adult donor, cultured in M2 Medium

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium (Ready-to-use): Includes phenol red-free Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial

Quality

All lots of PromoCell Melanocyte Growth Medium M2 are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Articles

Primary Human Epidermal Melanocytes and optimized growth media support in vitro cultivation.

Primary Human Epidermal Melanocytes and optimized growth media support in vitro cultivation.

Primary Human Epidermal Melanocytes and optimized growth media support in vitro cultivation.

Primary Human Epidermal Melanocytes and optimized growth media support in vitro cultivation.

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