This activity of this product is not tested on a lot-to-lot basis. However, historical data indicate a range of 0.28 - 0.40 units per milligram.
A9253
L-Amino Acid Oxidase from Crotalus adamanteus
Type I (dried venom)
Synonym(s):
L-AAO, L-Amino acid: oxygen oxidoreductase (deaminating)
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About This Item
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biological source
Crotalus adamanteus
Quality Level
type
Type I (dried venom)
Type I
form
powder
mol wt
~130 kDa
solubility
H2O: soluble 1.0 mg/mL, clear(lit.)
storage temp.
−20°C
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General description
L-Amino acid oxidase (LAAO) is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70 kDa.[1] Each molecule of holoenzyme has two flavin adenine dinucleotide (FAD) molecules.[2] LAAO is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. optimum pH is approximately 7.5.[3] It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney.[3] In the N-terminal region, it has a βαβ domain with glutamic acid residues.[3] LAAO imparts yellow color to venom.[4]
Application
L-Amino Acid Oxidase from Crotalus adamanteus has been used in the quantification of rid protein in imine deaminase activity.[5]
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays.[6] The enzyme has been immobilized and used in an enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine.[7]
Biochem/physiol Actions
L-Amino acid oxidase (LAAO) from Crotalus adamanteus requires Mg2+ for its activation.[3] The N-terminal region is essential for FAD binding.[3] The H2O2 generated by LAAO based oxidation reactions lead to prevention of platelet aggregation and induces edema and apoptosis.[4] LAAO activity is inhibited by ethylenediaminetetraacetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF).[8]
Preparation Note
Dissolves in water at 1 mg/mL concentration to form a clear solution.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Customers Also Viewed
Characterization of L-amino Acid Oxidase Derived from Crotalus adamanteus Venom: Procoagulant and Anticoagulant Activities
Nielsen VG
International Journal of Molecular Sciences, 20(19), 4853-4853 (2019)
The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)
Rokyta DR, et al.
BMC Genomics, 13(1), 312-312 (2012)
An enzymatic flow-injection procedure with chemiluminescence detection for on-site determination of L-alanine in synthesis process
Borges EP and Reis BF
Journal of the Brazilian Chemical Society, 16(6), 1226-1232 (2005)
Glycerol-induced development of catalytically active conformation of Crotalus adamanteus L-amino acid oxidase in vitro
Raibekas AA and Massey V
Proceedings of the National Academy of Sciences of the USA, 93(15), 7546-7551 (1996)
Alba Martín-Barreiro et al.
Analytical and bioanalytical chemistry, 414(8), 2641-2649 (2022-01-23)
An enzymatic-colorimetric method has been developed based on the reaction between L-phenylalanine (L-Phe) and the L-amino acid oxidase (LAAO) in the presence of Au(III), which has led to the formation of gold nanoparticles. The intensity of the localized surface plasmon
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