MAB4234
Anti-Wilms′ Tumor Antibody, NT, clone 6F-H2
clone 6F-H2, Chemicon®, from mouse
Synonym(s):
WT1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F-H2, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WT1(7490)
General description
Wilms tumor protein (UniProt P19544; also known as WT33) is encoded by the WT1 (also known as DDS, FS, MEACHS, MESOM, NPHS4, WAGR) gene (Gene ID 7490) in human. The Wilms’ tumor gene WT1 was originally identified in the childhood kidney cancer Wilms’ tumor. The N-terminal region of WT1 protein contains a proline-rich region (a.a. 27-83) involved in transcriptional regulation, self-association, and RNA recognition, while its C-terminal region contains four zinc fingers (a.a 323-347, 353-377, 383-405, 414-438) that mediate DNA and RNA binding. The zinc finger domain of WT1 can bind to GC-rich sequences, such as the EGR-1 consensus sequence (5’-GCG(T/G)GGGCG-3’), the WTE motif (5′-GCGTGGGAGT-3′), or (TCC)n motif. Many genes responsible for cell growth and apoptosis, such as Bcl-2, Bcl-xL, BFL1, and c-myc, have been identified as downstream targets of WT1. There are four major alternatively spliced WT1 isoforms resulting from splicing at either or both of exon 5 (17AA) and exon 9 (KTS). All four major WT1 isoforms are overexpressed in leukemia and solid tumors and play oncogenic roles such as inhibition of apoptosis, and promotion of cell proliferation, migration and invasion.
Specificity
Expected to react with all spliced isoforms other than isoforms 6 and 9 reported by UniProt (P19544). Reactivity toward isoforms 6 and 9 has not been determined.
Immunogen
Epitope: Amino acids 1-173.
Recombinantt human WT-1 N-terminal fragment (a.a. 1-173).
Application
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CRE-binding protein/CBP together with Wilms tumor protein WT1 from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in the CRE-binding protein/CBP immunoprecipitate obtained from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in lysates from mouse E15.5 embryonic kidney and human melanoma cell lines A375, SK-MEL-28, and WM-266-4 (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of methanol-fixed human melanoma A375 cells by fluorescent immunocytochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunofluorescence Analysis: A representative lot immunostained the PCNA-positive nuclei of proliferating cells in formalin-fixed, paraffin-embedded human melanoma tissue sections by fluorescent immunohistochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunohistochemistry Analysis: A representative lot immunostained glomeruli in formalin-fixed, paraffin-embedded normal human kidney and Wilms′ tumor sections (Wagner, N., et al. (2008). Pediatr. Nephrol. 23(9):1445-1453).
Immunohistochemistry Analysis: A representative lot detected vascular WT1 expression in 95% of 113 paraffin-embedded tumour tissues of various types. In most cases, nuclear WT1 staining of endothelial cells was seen (Wagner, N., et al. (2008). Oncogene. 27(26):3662-3672).
Immunohistochemistry Analysis: A representative lot immunostained the nucleus of perifollicular fibroblasts at the hair follicle in formalin-fixed, paraffin-embedded normal human skin sections. Most common melanocytic nevi do not express WT1, whereas Spitz nevi and dysplastic nevi show cytoplasmic WT1 staining. (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in the CRE-binding protein/CBP immunoprecipitate obtained from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in lysates from mouse E15.5 embryonic kidney and human melanoma cell lines A375, SK-MEL-28, and WM-266-4 (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of methanol-fixed human melanoma A375 cells by fluorescent immunocytochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunofluorescence Analysis: A representative lot immunostained the PCNA-positive nuclei of proliferating cells in formalin-fixed, paraffin-embedded human melanoma tissue sections by fluorescent immunohistochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunohistochemistry Analysis: A representative lot immunostained glomeruli in formalin-fixed, paraffin-embedded normal human kidney and Wilms′ tumor sections (Wagner, N., et al. (2008). Pediatr. Nephrol. 23(9):1445-1453).
Immunohistochemistry Analysis: A representative lot detected vascular WT1 expression in 95% of 113 paraffin-embedded tumour tissues of various types. In most cases, nuclear WT1 staining of endothelial cells was seen (Wagner, N., et al. (2008). Oncogene. 27(26):3662-3672).
Immunohistochemistry Analysis: A representative lot immunostained the nucleus of perifollicular fibroblasts at the hair follicle in formalin-fixed, paraffin-embedded normal human skin sections. Most common melanocytic nevi do not express WT1, whereas Spitz nevi and dysplastic nevi show cytoplasmic WT1 staining. (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
This Anti-Wilms′ Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms′ tumor protein.
Target description
~52 kDa observed. 49.19 kDa (isoform 1), 47.20 kDa (isoform 2), 47.51 kDa (isoform 3), 48.87 kDa (isoform 4), 34.45 kDa (isoform 5), 56.88 kDa (isoform 6), 55.21 kDa (isoform 7), 33.09 kDa (isoform 8) calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein A purified.
Purified mouse IgG1κ in 0.02 M phosphate buffer (pH 7.6), 0.25 M NaCl and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rita Carmona et al.
eLife, 5 (2016-09-20)
Congenital diaphragmatic hernia (CDH) is a severe birth defect. Wt1-null mouse embryos develop CDH but the mechanisms regulated by WT1 are unknown. We have generated a murine model with conditional deletion of WT1 in the lateral plate mesoderm, using the
Xingrui Mou et al.
Bioengineering (Basel, Switzerland), 9(5) (2022-05-28)
Podocytes derived from human induced pluripotent stem (hiPS) cells are enabling studies of kidney development and disease. However, many of these studies are carried out in traditional tissue culture plates that do not accurately recapitulate the molecular and mechanical features
Nan Zang et al.
iScience, 25(10), 105145-105145 (2022-10-01)
Diabetic kidney disease (DKD) is the leading cause of end-stage renal diseases. DKD does not have efficacious treatment. The cGAS-STING pathway is activated in podocytes at the early stage of kidney dysfunction, which is associated with the activation of STING
Elena Cano et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(3), 656-661 (2016-01-08)
Recent reports suggest that mammalian embryonic coronary endothelium (CoE) originates from the sinus venosus and ventricular endocardium. However, the contribution of extracardiac cells to CoE is thought to be minor and nonsignificant for coronary formation. Using classic (Wt1(Cre)) and previously
Samira Musah et al.
Nature biomedical engineering, 1 (2017-10-19)
An in vitro model of the human kidney glomerulus - the major site of blood filtration - could facilitate drug discovery and illuminate kidney-disease mechanisms. Microfluidic organ-on-a-chip technology has been used to model the human proximal tubule, yet a kidney-glomerulus-on-a-chip
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