EZRMI

Rat/Mouse Insulin ELISA Kit

measures and quantifies insulin levels in 10 μL serum or plasma

• Synonym(s): Insulin
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Properties

• product name: Rat/Mouse Insulin ELISA, This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
• Quality Level: 200
• species reactivity: mouse, rat
• packaging: kit of 1 × 96 wells
• parameter: 10 μL sample volume (4hr assay)
• assay range: accuracy: 83-102%; linearity: 79-133%; sensitivity: 0.2 ng/mL; standard curve range: 0.2-10 ng/mL
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma (K2 EDTA)
• UniProt accession no.: P01322; P01325
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Insulin is secreted by the islet β cells in the pancreas. It contains two peptide chains linked by disulfide bridges. The A chain is composed of 21 amino acids and the B chain has 30 amino acids.

Application

Rat/Mouse Insulin ELISA is suitable for measuring and quantifying insulin levels in metabolism and endocrine research. It has been used to determine plasma insulin by enzyme-linked immunosorbent assay. It has also been used to measure the insulin secretion from cells, in vitro.

Biochem/physiol Actions

Insulin is a key hormone that maintains normal blood glucose levels. It mediates cellular glucose uptake, controls carbohydrate, lipid, and protein metabolism, and enhances cell division and growth through its mitogenic effects. Insulin resistance is a condition of impaired sensitivity to insulin-mediated glucose disposal.

Other Notes

3 hour assay, room temperature 10 μL sample size
Research Category: Metabolism
Research Sub Category: Obesity/Metabolic Disorders

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Warning
• Hazard Statements: H290,H317
• Precautionary Statements: P234 - P261 - P272 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials