EQCYTMG-93KPX23

MILLIPLEX^® Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: horse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 76%; (IL-6); accuracy: 78%; (FGF-2); accuracy: 82%; (G-CSF); accuracy: 85%; (IL-13); accuracy: 87%; (TNFα); accuracy: 88%; (RANTES/CCL5); accuracy: 90%; (IL-2); accuracy: 92%; (GM-CSF); accuracy: 94%; (IL-18); accuracy: 97%; (GRO); sensitivity: 1.4 pg/mL; (IL-6); sensitivity: 10.6 pg/mL; (IL-5); sensitivity: 13.5 pg/mL; (IL-2); sensitivity: 15.4 pg/mL; (IL-17A); sensitivity: 16.3 pg/mL; (FGF-2); sensitivity: 16.7 pg/mL; (IP-10); sensitivity: 165 pg/mL; (IFNγ); sensitivity: 25.3 pg/mL; (IL-18); sensitivity: 29.3 pg/mL; (IL-1β); sensitivity: 3.9 pg/mL; (RANTES/CCL5); sensitivity: 3.94 pg/mL; (GM-CSF); sensitivity: 340 pg/mL; (G-CSF); sensitivity: 355 pg/mL; (IL-4); sensitivity: 39-130 pg/mL; (IL-13); sensitivity: 39.1 pg/mL; (IL-1α); sensitivity: 399 ng/mL; (MCP-1); sensitivity: 457 pg/mL; (Fractalkine/CS3CL1); sensitivity: 49.2 pg/mL; (IL-12 (p70)); sensitivity: 5.2 pg/mL; (TNFα); sensitivity: 62.5 pg/mL; (IL-10); sensitivity: 7.8 pg/mL; (GRO); sensitivity: 74.3 pg/mL; (IL-8/CXCL8); sensitivity: 8.3 pg/mL; (Eotaxin/CCL11); standard curve range: 15-15,000 pg/mL; (IL-17A); standard curve range: 15-15,000 pg/mL; (IL-2); standard curve range: 15-15,000 pg/mL; (IL-5); standard curve range: 15-15,000 pg/mL; (IL-6); standard curve range: 15-15,000 pg/mL; (IP-10); standard curve range: 24-25,000 pg/mL; (FGF-2); standard curve range: 29-30,000 pg/mL; (IL-1β); standard curve range: 293-300,000 pg/mL; (IL-4); standard curve range: 34-35,000 pg/mL; (IL-18); standard curve range: 342-350,000 ng/mL; (MCP-1); standard curve range: 39-40,000 pg/mL; (IL-12 (p70)); standard curve range: 39-40,000 pg/mL; (IL-13); standard curve range: 4-4,000 pg/mL; (TNFα); standard curve range: 488-500,000 pg/mL; (IFNγ); standard curve range: 49-50,000 pg/mL; (IL-10); standard curve range: 5-5,000 pg/mL; (GM-CSF); standard curve range: 5-5,000 pg/mL; (RANTES/CCL5); standard curve range: 586-600,000 pg/mL; (Fractalkine/CS3CL1); standard curve range: 59-60,000 pg/mL; (IL-1α); standard curve range: 59-60,000 pg/mL; (IL-8/CXCL8); standard curve range: 6-6,000 pg/mL; (GRO); standard curve range: 7-7,000 pg/mL; (Eotaxin/CCL11); standard curve range: 78-80,000 pg/mL; (G-CSF)
• technique(s): multiplexing: suitable
• compatibility: configured for Premixed
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for horses as it is for humans. Both experience immunological diseases, such as infectious diseases, osteoarthritis, and respiratory diseases, as well as neurologic, metabolic, and cardiovascular diseases and cancer. Until now the ability to measure levels of cytokines and chemokine in horses had been limited primarily to ELISAs. Researchers can now measure multiple inflammation biomarkers simultaneously using the MILLIPLEX^® Equine Cytokine/Chemokine Panel, based on the Luminex^® xMAP^® technology.

MILLIPLEX^® Equine Cytokine/Chemokine Premix Panel is a 23-plex kit used for the simultaneous quantification of all the following analytes in equine serum, plasma, or culture supernatant samples: Eotaxin/CCL11, FGF-2, Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

• Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α
• Recommended Sample Type: Equine serum, plasma, tissue culture supernatant or tissue/cell extracts
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require dilution with an appropriate medium
• Assay Run Time: Overnight or One Day A primary incubation of 2 hours at room temperature (RT) (20-25°C), or for greater sensitivity, overnight (16-18 hours) at 2-8°C; followed by a 1 hour secondary incubation at RT
• Research Category: Inflammation & Immunology

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 10 - Combustible liquids