ABE154
Anti-Histone H3.1/H3.2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3.1 and H3.2 are closely related histone variants that differ by only one amino acid. Mammalian cells express three major types of noncentromeric histone H3 variants, H3.1, H3.2, and H3.3. H3.1 is only expressed in mammals, while H3.2 is found in all eukaryotes except for yeast. Recent evidence shows that these nearly identical histone proteins differ in their expression profiles and post-translational modifications (PTMs). H3.2 is enriched in modifications associated with transcriptional silencing (di- and tri-methylation of K27), whereas H3.1 is enriched in both K9 dimethylation and K14 acetylation, suggesting that these variants may have separate biological functions.
Specificity
This antibody is expected to demonstrate broad species reactivity.
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3.1 and H3.2.
Application
Anti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blotting Analysis: A representative lot detected Histone H3.2 in recombinant Histone H3.2 and mouse Embryonic Stem cell lysates.
Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
Quality
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract.
Target description
~17 kDa observed. Uncharacterized bands may be observed at ~25 kDa and ~50 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa acid extract
HeLa acid extract
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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