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Key Documents

AB3282

Sigma-Aldrich

Anti-Glutathione-S-Transferase Antibody, S. japonicum form

Chemicon®, from rabbit

Synonym(s):

GST-tag

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Schistosoma japonicum, vertebrates

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GSTP1(2950)

Specificity

Anti-Glutathione S-transferase (GST) is a polyclonal antibody to the recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.

The anti GST antibody reacts specifically with the glutathione S-transferase moiety of GST fusion proteins as expressed in E.coli by the pGEX (1) vectors and in yeast by the pYEX(TM) vectors (2).


(1)The pGEX expression vector system is a plasmid and purification system which, in most cases, results in the fusion protein being soluble in aqueous solutions for extraction and purification from crude bacterial lysates. This expression vector system enables the protein glutathione S-transferase (GST) to be produced in fusion with the protein of interest so as to bind with high affinity to a glutathione affinity column. The GST-protein complex can then be easily purified by elution from the column with reduced glutathione. The pGEX vectors are available as the GST System from Pharmacia Biotech.

(2) pYEX is a yeast GST expression vector supplied by AMRAD biotech of Australia.

Immunogen

Epitope: S. japonicum form
Recombinant glutathione S-transferase (GST) protein from Schistosoma japonicum.

Application

Anti-Glutathione-S-Transferase Antibody, S. japonicum form is an antibody against Glutathione-S-Transferase for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
The anti-GST antibody can be used to detect GST fusion proteins in Western blot analysis and ELISA studies.

Physical form

Format: Purified
Liquid in PBS, pH 7.4, containing 0.02% Tween 20 and 0.02% sodium azide as a preservative.

Storage and Stability

The anti GST antibody is shipped liquid at 4°C. The anti GST antibody is stable for up to 6 months when stored at 2-8°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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