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AB1620

Sigma-Aldrich

Anti-Vimentin Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

NM_003380.2

UniProt accession no.

P08670

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

Specificity

Recognizes vimentin in cultured mammalian cells and tissue sections. AB1620 specifically stains the vimentin band (58 kD) using total cell or tissue extracts in immunoblotting. No reactivity to desmin, keratin, neurofilaments or glial filaments. Shows wide cross-reactivity among mammalian species using various immunolabeling techniques.

Immunogen

Vimentin purified from cultured human foreskin fibroblasts.

Application

Anti-Vimentin Antibody detects level of Vimentin & has been published & validated for use in IC, IH, IH(P) & WB.
Immunohistochemistry: 1:20-1:40. Shows reactivity on frozen tissue sections as well as formalin-fixed paraffin embedded tissue by immunoperoxidase and immunofluorescent assays.

Immunocytochemistry

Western blot

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Target description

58 kDa

Physical form

Neat goat Serum. Liquid containing 0.1% sodium azide.
Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Breast carcinoma tissue, Colon, Prostate, Tonsil, Tonsilar lymphoma, Uterus, Ureter

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kotula, E; Faigle, W; Berthault, N; Dingli, F; Loew, D; Sun, JS; Dutreix, M; Quanz, M
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Sebastian E Illanes et al.
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