T9378
Trypsin inhibitor
crude powder
Synonyme(s) :
Trypsin Inhibitor from lima beans
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Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
biological source
Phaseolus limensis (lima bean)
Quality Segment
type
Type II-L
form
crude powder
mol wt
9 kDa
input
plant, fungi and insect sample(s)
solubility
0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
storage temp.
2-8°C
General description
Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.
Application
Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
- in the inhibition of trypsin in human plasma
- in the termination of proteolytic digestion in rabbit skeletal muscles
- in the inhibition of salivary glands enzyme extract from Lygus lineolaris
- in the inhibition of proteolytic activity in Agave tequilana enzyme extract
Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.
Biochem/physiol Actions
Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.
Preparation Note
Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).
Analysis Note
One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.
Other Notes
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
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Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Classe de stockage
11 - Combustible Solids
Que se passe-t-il ?
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
EPI (équipement de protection individuelle)
Eyeshields, Gloves, type N95 (US)
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