T9285

Tampon Tris-EDTA

100X concentrate for Northern and Southern blotting

• Synonyme(s) : Tampon TE solution
• NACRES: NA.25
• PubChem Substance ID: 329826904
• UNSPSC Code: 41105319
• MDL number: MFCD00236359

Propriétés

• grade: Molecular Biology
• Quality Segment: 200
• sterility: 0.2 μm filtered
• form: liquid
• pH: 7.8-8.2 (1 × in water)
• application(s): sample preparation
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• InChI key: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Description

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Preparation Note

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

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Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable