T7693
Trizma® Pre-set crystals
BioPerformance Certified, pH 7.4, average Mw 151.6
Synonyme(s) :
Tris crystals
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A propos de cet article
NACRES:
NA.25
UNSPSC Code:
12161700
Service technique
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BioPerformance Certified
Quality Segment
form
crystalline powder
mol wt
average Mw 151.6
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)
pH
7.40 (25 °C, 0.1 M)(lit.), 7.4
useful pH range
7.0-9.0
solubility
water: 0.66 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
Application
Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Preparation Note
Trizma solutions can be autoclaved.
Other Notes
Certain pH electrodes do not give accurate results with Tris buffers.
This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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