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T0956

Sigma-Aldrich

Sigma Universal Transfection Reagent

Polymer reagent for transfecting common mammalian cell lines

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About This Item

Code UNSPSC :
12352202

Qualité

for molecular biology

Niveau de qualité

200

Stérilité

aseptically filled

Forme

liquid (buffered aqueous solution)

Utilisation

sufficient for 110 transfections (3.5 cm dish)
 sufficient for 40 transfections (10 cm dish)
 sufficient for 65 transfections (6 cm dish)

Concentration

2 mg/mL

Température de stockage

−20°C

Description générale

Sigma Universal Transfection Reagent is a unique formulation of a biodegradable, proprietary cationic polymer conjugated to a cationic lipid. Universal Transfection Reagent is used for transfection of nucleic acids into common eukaryotic cell lines. Fast and easy protocol is compatible with serum, serum-free medium and antibiotics. For most cells, medium change after transfection is not necessary.

Application

Suitable for transient and stable transfection of nucleic acids into cultured eukaryotic cells. Use approximately 1-3 μl Universal Transfection Reagent per μg DNA. Protocol optimization provides very efficient transfection. The following cells have been successfully transfected using Sigma Universal Transfection Reagent:

Excellent for:
BHK-21
CHO
CHOK1
HEK293
HeLa
NIH3T3

Good for:
3LL
6CSFME
ATT20B16-F0
B16-F10
BAEC
Ca Ski
COS-7
CV-1
D 407
HUVEC
L929
MCF-7
MDCK
Neuro2A
SAOS-2
SHEP
SiHa
SKOV3

Caractéristiques et avantages

  • Fast, easy protocol
  • Suitable for stable and transient transfection
  • High efficiency for a wide variety of cell types including primary cells
  • Compatible with both serum and serum-free transfection protocols

Composants

Note the formulation of Sigma Universal Transfection Reagent is confidential.

Principe

Sigma Universal Transfection Reagent is a cationic polymer conjugated to a cationic lipid. When mixed with plasmid DNA, the reagent forms stable complexes that can be directly added to the cell culture. The cells take up the complexes by endocytosis, the polymer is cleaved from the complex, and the plasmid DNA is quickly released into the cytoplasm.

Autres remarques

Complex formation is inhibited by serum, but once stable complexes have formed, the presence of serum lowers the toxicity of transfection.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

CAPHOS

Calcium Phosphate Transfection Kit, Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

NPT01

NeuroPorter™ Transfection Kit, Lipid formulation for nucleic acid transfections in neuronal and glial cells

XTG9-RO

Réactif de transfection d'ADN X-tremeGENE™ 9, Polymer reagent for transfecting common cell lines

XTGHP-RO

Réactif de transfection d'ADN X-tremeGENE™ HP, High-performance polymer reagent for transfecting many cell lines

SITRAN-RO

X-tremeGENE™ siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Pan Pan et al.
Nature communications, 12(1), 4664-4664 (2021-08-04)
Excessive inflammatory responses induced upon SARS-CoV-2 infection are associated with severe symptoms of COVID-19. Inflammasomes activated in response to SARS-CoV-2 infection are also associated with COVID-19 severity. Here, we show a distinct mechanism by which SARS-CoV-2 N protein promotes NLRP3

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