SCA20
Sucrose Assay Kit
sufficient for 20 assays
Synonyme(s) :
Sucrose Test Kit
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About This Item
Produits recommandés
Utilisation
sufficient for 20 assays
Niveau de qualité
Classe(s) chimique(s) de l'analyte
sugars (sucrose)
Technique(s)
photometry: suitable
Application(s)
food and beverages
general analytical
Température de stockage
2-8°C
Description générale
Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.
Application
This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.
Composants de kit seuls
Réf. du produit
Description
- glucose assay reagent 50 mL
- sucrose assay reagent 1 mL/vial
- sucrose standard 500 mg
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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