S7651

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

• Synonyme(s) : Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• Numéro CAS: 9031-54-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-869-9
• MDL number: MFCD00132362
• Numéro CE : 3.1.4.12 (BRENDA, IUBMB)
• Specific activity: ≥100 units/mg protein

Propriétés

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥100 units/mg protein
• mol wt: 36873 Da
• composition: Protein, ~10% Lowry
• storage temp.: 2-8°C
• Gene Information: Bacillus cereus ATCC 14579 ... BC0671(1203022)

Description

General description

Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages. Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg²⁺-containing metalloenzyme. Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.

Application

Sphingomyelinase from Bacillus cereus has been used:

• in the measurement of membrane capacitance in brain slices
• for testing and confirming its silkworm-killing activity
• in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): Eyeshields, Gloves, type N95 (US)
• Pictogrammes: GHS08
• Mot-clé: Danger
• Codes de danger: H334
• Mentions de précaution: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Que se passe-t-il ?: WGK 1