Name: Custom qPCR Probes
Brand: SIGMA

Custom DNA and RNA Oligos & qPCR Probes Product Resources

Custom Oligos & qPCR Probes Online Tools

<h2>&nbsp;</h2>
<h2>Dual-Labeled Probes</h2>


<h2>Black Hole Quencher™&nbsp;(BHQ™)</h2>


<ul>
<li>Amounts: 1, 3, 5, and 10 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Comprehensive offering of reporters and quenchers</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/molecular-beacons">Learn more</a></li>
</ul>


<ul>
<li>Available with Dual-Labeled Probes and other oligos</li>
<li>Increased thermal stability and hybridization specificity</li>
<li>Easier and more specific designs for problematic target sequences</li>
<li><a href="/technical-documents/technical-article/genomics/pcr/locked-nucleic-acids-faq">Learn more</a></li>
</ul>


<ul>
<li>Available for all Custom qPCR Probe types</li>
<li>No native fluorescence (emits heat instead of light), resulting in lower background fluorescence</li>
<li>Increased signal-to-noise ratio, providing higher sensitivity</li>
<li>Enables a wider choice of reporters for multiplexing</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/black-hole-quenchers">Learn more</a></li>
</ul>


<ul>
<li>Amounts: 1, 3, 5, and 10 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid and MGB:EDQ available</li>
<li>Dyes:&nbsp;Comprehensive offering of reporters and 3' / internal / dual quenchers</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/dual-labeled-probes">Learn more</a></li>
</ul>
<p>&nbsp;</p>


<ul>
<li>Amounts: 0.1, 0.25, 1.5, and 15 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/lightcycler-probes">Learn more</a></li>
</ul>


<h2>Locked Nucleic Acid &amp; MGB:EDQ</h2>


<h2>LightCycler<sup>®</sup>&nbsp;Probes</h2>


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Custom qPCR Probes Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR; Synonyms: BHQ,BHQ Probes,Black Hole Quencher,Dual-Quenched Probes,,Internal-Quenched Probes,Locked Nucleic Acid probes,MGB:EDQ Probes,Probes,Real-Time PCR Probes,locked nucleic acid,qPCR assays,qPCR designs at Sigma-Aldrich

Custom qPCR Probes Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR | Sigma-Aldrich

Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR

Custom qPCR Probes Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

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Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, MGB:EDQ and BHQ Probes for qPCR

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